Table 1.
Details of the lepidopteran mitogenomes used in this study.
Table 2.
Details of the primers used to amplify the mitogenome of D. nerii.
Fig 1.
Map of the mitogenome of D.nerii.
The tRNA genes are labeled according to the IUPAC-IUB single-letter amino acids: cox1, cox2 and cox3 refer to the cytochrome c oxidase subunits; cob refers to cytochrome b; nad1-nad6 refer to NADH dehydrogenase components; rrnL and rrnS refer to ribosomal RNAs.
Table 3.
List of annotated mitochondrial genes of D. nerii.
Table 4.
Composition and skewness in different Lepidopteran mitogenomes.
Fig 2.
Comparison of codon usage within the mitochondrial genome of members of the Lepidoptera.
Lowercase letters (a, b, c, d and e) above species name represent the superfamily to which the species belongs (a: Bombycoidea, b: Noctuoidea, c: Tortricoidea, d: Hesperioidea, e: Geometroidea).
Fig 3.
Codon distribution in members of the Lepidoptera.CDspT = codons per thousand codons.
Fig 4.
The Relative Synonymous Codon Usage (RSCU) of the mitochondrial genome of six superfamilies in the Lepidoptera.
Codon families are plotted on the X axis. Codons indicated above the bar are not present in the mitogenome.
Fig 5.
Putative secondary structures of the 22 tRNA genes of the D.nerii mitogenome.
Fig 6.
Alignment of overlapping region between atp8 and atp6 across Lepidoptera and other insects.
The numbers on the right refer to intergenic nucleotides.
Fig 7.
(A) Alignment of the intergenic spacer region between trnS2 (UCN) and nad1 of several Lepidopteran insects. The shaded ‘ATACTAA’ motif is conserved across the Lepidoptera order. (B) Features present in the A+T-rich region of D.nerii. The sequence is shown in the reverse strand. The ATATG motif is shaded. The poly-T stretch is underlined while the poly-A stretch is double underlined. The single microsatellite T/A repeats sequence are indicated by dotted underlining.
Fig 8.
Tree showing the phylogenetic relationships among Lepidopteran insects, constructed using (A) Bayesian inference (BI). (B) Maximum Likelihood method (ML). Bootstrap values (1000 repetitions) of the branches are indicated. Drosophila melanogaster (U37541.1) and Locustamigratoria (NC_001712) were used as outgroups.