Fig 1.
Overview of sample collection.
H&E stained lymph nodes 9, 14, 15, 16 and 19 after microdissection with the highlighted areas from which the microdissections of ISFN-containing follicles were performed. The holes caused by microdissection are in part clearly visible.
Fig 2.
IGH clonality analysis of analysed follicles.
A) Electropherograms of VDJ rearrangement clonality analysis using the framework 2 (FR2) primer set show a clonal product of 268 base pairs size. DNA from complete lymph node section. B) Electropherograms of six microdissected samples containing single or pooled ISFN lesions which showed products by fragment analysis based PCR amplification. Green arrows indicate the clonal 268 base pair products.
Fig 3.
Identification of ISFN-associated reads and read groups.
A) Table of the samples according to their total reads, percentage of specific and nonspecific reads and their groups. B) Numbers of specific reads which were identified in the different samples. Productive rearrangements are depicted as coloured bars indicating the assignment to the cluster groups; grey bars indicate unproductive rearrangements. In five samples no specific reads were identified. C) All specific reads clustered into 11 groups of identical CDR3 amino acid sequence based on sequence similarities. Columns indicate the number of respective sequences which are assigned to a group.
Fig 4.
Phylogenetic tree of the eleven sequence groups.
Spatial distance of the groups corresponds to the number of diverging base pairs. Dotted line indicates further distant relation to the three single sequences, which could not be assigned to the eleven groups.