Table 1.
Clinical characteristics.
Table 2.
Selected pathways over-represented among differentially expressed genes between OIR and OIS womena.
Table 3.
Glucose uptake and insulin signaling genes differentially expressed between OIR and OIS women.
Fig 1.
Flowchart describing the project.
Table 4.
Genes differentially expressed between OIR and OIS women with FDR ≤5% and fold change in expression 20%.
Fig 2.
Effects of KLF15 and SLC25A10 knockdownon lipogenesis in vitro.
A. KLF15 and SLC25A10 were knocked down using 40nM of siRNA in SVF-derived human adipocytes differentiated in vitro and expression of the genes evaluated using real-time PCR. Results were analyzed using Students t-test and are presented as relative fold change ± SD vs. negative control. B. SVF-derived adipocytes differentiated in vitro were transfected with 40 nM of siRNA against KLF15 and SLC25A10 for 48 hours followed by evaluation of basal and insulin-stimulated lipogenesis. Relative insulin-stimulated lipogenesis was calculated against non-targeting siRNA NegC at insulin-stimulated state. Induction of lipogenesis by insulin for NegC was minimum 3-fold in all experiments. Results are based on three to five biological/independent experiments.*p<0.05, **p<0.01 and ***p<0.001.
Fig 3.
Ingenuity analysis of KLF15 target genes associated with insulin stimulated lipogenesis in vivo.
Table 5.
Gene sets enriched among target genes in KLF15 knockdown.
Table 6.
Genes associated with adipocyte IR also associated with systemic insulin resistance or T2D.