Fig 1.
Phenotypic, biochemistry analysis of G. klotzschianum in response to salt stress.
A: Phenotype of G. klotzschianum and G. davidsonii post salt stress (200 mM, 250 mM, 300 mM NaCl) for three days. B a-d: the changes of ABA, ETH, GA, and IAA concentration at different times post 300 mM NaCl stress. C a-d: the changes of GSSH, GSH, H2O2, and AsA concentration at different times post 300 mM NaCl stress. G. klotzschianum seedlings grown under normal conditions were used as controls. Three biological replicates were used. Multiple comparisons were performed with significant difference in different letter at P < 0.05 level; Error bars represent SD.
Table 1.
Summary of RNA-Seq results and their matches in the G. raimondii genome.
Fig 2.
Summary of DEGs in leaves and roots of G. klotzschianum upon salt stress.
A: Number of DEGs by salt stress under different time points (L3 GKLS3 vs. GKLC3, L12 GKLS12 vs. GKLC12, L48 GKLS48 vs. GKLC48, R3 GKRS3 vs. GKRC3, R12 GKRS12 vs. GKRC12, R48 GKRS48 vs. GKRC48), in leaves and roots. B: Comparion of up-/down-regulated genes in roots and leaves. C: Number of regulated genes between different conditions (LSC LS vs. LC, RSC RS vs. RC).
Fig 3.
A: A heat map of 1406 differentially expressed genes from seven root samples. B: A heat map of 782 differentially expressed genes from seven leaf samples.
Fig 4.
qRT-PCR validation of transcript levels evaluated by RNA-Seq in roots and leaves of G. klotzschianum under 300 mM NaCl stress conditions.
(A, B) represent the correlation of the fold change analyzed by RNA-Seq platform (x-axis) with data obtained using real time PCR (y-axis) in roots and leaves of G. klotzschianum. 19 independent genes for each organ were randomly selected, three biological replicates were used from each sample at three time points post salt stress.
Fig 5.
A: GO functional classification of DEGs in roots. B: GO functional classification of DEGs in leaves.
Table 2.
GO terms related to stimulus or stress of DEGs.
Fig 6.
Hormones and ROS-related pathways under salt stress.
Fig 7.
Expression profiling of hormone synthesis (A), hormone transduction (B), ROS (C), ion homeostasis (D) related genes.
Fig 8.
Heatmap analysis of the expression levels of ABA, ROS and SOS-related genes.
A: A Heatmap by qRT-PCR Log2 Fold Change in roots and leaves of G. klotzschianum. B: A Heatmap by RNA-Seq Log2 Fold Change in roots and leaves of G. klotzschianum. The results were performed by the Fold Change ≥2.
Fig 9.
Hypothetical interaction network at early stage in response to salt stress in cotton.
Black arrow: salt stress induced hormone biosynthesis and signal transduction, likewise, the SOS pathway in the early part of the treatment, which leads to H2O2 accumulation; Blunt arrow: negative regulation.