Fig 1.
Flow chart of synthesis of 2DG gold nanoparticle at room temperature method.
Fig 2.
The reduction reaction equation for the formation of Au nanoparticles.
Fig 3.
UV-visible absorption spectrum of AuNPs.
A UV-visible absorption spectrum of glucose-AuNps showing SPR peak at 540 nm; B Citrate-AuNps showing SPR peak at 520 nm; C 2DG-AuNps showing SPR peak at 525 nm.
Fig 4.
FTIR and SERS spectrum analysis.
FTIR spectrum of (A) glucose and glucose-gold nanoparticles; (B) 2DG and 2DG-gold nanoparticles; C: SERS Raman spectra of 2DG-Au and Raman spectra of 2DG.
Fig 5.
5A, 5C High magnification TEM image of glucose capped gold nanoparticles showing FFT measurement using Image-J software; 5B SAED pattern of glucose capped gold nanoparticles; 5D, 5F High magnification TEM image of citrate -gold nanoparticles showing FFT measurement using Image J-software; 5E SAED pattern of citrate—gold nanoparticles; 5 G, 5I Shows the high magnification TEM image of 2-Deoxy-D-Glucose capped gold nanoparticles;5 H SAED pattern of 2DG-Capped gold nanoparticles.
Fig 6.
Size distribution measurement plot of AuNPs.
Size distribution measurement curve of (A) glucose-gold nanoparticles; (B) citrate-gold nanoparticles; C Dynamic light scattering measurement of 2DG coated gold of size 21.51nm.
Fig 7.
SEM-EDS spectrum of glucose-capped gold nanoparticles and 2DG-capped gold nanoparticles.
SEM-EDS analysis of (A) glucose-capped gold nanoparticles; (B) 2DG-capped gold nanoparticles;
Fig 8.
Cell viability plot and dendrogram plot.
A Viability of HeLa cells, HepG2 cells and HCT 116 cells treated with 2-Deoxy-D-Glucose-gold nanoparticles; B Viability of HeLa cell line; HepG2 cell line and HCT116 cells treated with glucose capped gold nanoparticles; C Viability of HeLa cells, HepG2 cells and HCT 116 cells treated with citrate-gold nanoparticles. Standard deviation shown as error bar, p<0.05. D: Dendrogram shows cell lines are aligned separately and grouped based on similarities in their cytotoxicity expression using a hierarchical clustering analysis technique. 1 represents 2DG-AuNp-Hela,2: 2DG-AuNp-HepG2, 3:2DG-AuNp-HCT116, 4:Glu-AuNp-Hela,5:Glu-AuNp-HepG2,6: Glu-AuNp-HCT116,7: Citr-AuNp-Hela,8: Citr-AuNp-HepG2, 9: Citr-AuNp-HCT116.