Table 1.
Specimens and sequences included in this study.
Fig 1.
The 50% majority-rule consensus tree of ITS/LSU nrDNA sequences of Myriostoma species using a Bayesian approach.
Two sequences of Geastrum saccatum were used as outgroup. Terminal branches are labelled with appropriate specimen codes and countries of origin. For further specimen details, see Table 1. Numbers at the nodes indicate the percentage bootstrap values obtained from parsimony and maximum likelihood analysis and the posterior probabilities from Bayesian analysis (MPbs/MLbs/PP).
Table 2.
Matrix of pairwise Kimura-2-parameter (K2P) distance between ITS sequences from the four species analysed in this paper.
Fig 2.
Myriostoma areolatum (MA-Fungi 68596, isotype).
(a) Dried expanded basidioma ex situ, bar = 10 mm. (b) Stomata, bar = 5 mm. (c) Basidiospores under SEM, bar = 2 μm. (d) Endoperidial surface under SEM, bar = 50 μm.
Fig 3.
(a) Fresh expanded and unexpanded basidiomata in situ (UFRN-Fungos 2019, holotype), bar = 20 mm. (b) Endoperidial surface (UFRN-Fungos 386, paratype), bar = 1 mm. (c) Stoma (UFRN-Fungos 386, paratype), bar = 2 mm. (d) Basidiospores under LM (UFRN-Fungos 2020, isotype), bar = 10 μm. (e) Capillitium under LM (UFRN-Fungos 2019, holotype), bar = 10 μm. (f) Basidiospores under SEM, bar = 2 μm. (g) Endoperidial surface under SEM (UFRN-Fungos 2019, holotype), bar = 50 μm.
Fig 4.
(a–b) Dried expanded basidiomata ex situ (KM205483 and K(M)205482, respectively), bar = 20 mm. (c–d) Basidiospores under LM (K(M)205483), bar = 10 μm. (e) Basidiospores under SEM (K(M)205483), bar = 2.5 μm. (f) Endoperidial surface under SEM (K(M)205483), bar = 50 μm.
Fig 5.
(a–b) Dried expanded basidiomata ex situ (K(M)138625, epitype), bar 10 mm. (c) Endoperidial surface (PC0723885), bar = 1 mm. (d) Stoma (MJ8714), bar = 1 mm. (e) Basidiospores under LM (K(M)138625, epitype), bar = 10 μm. (f) Capillitium under LM (MJ8714), bar = 10 μm. (g) Basidiospores under SEM (K(M)138625, epitype), bar = 2.5 μm. (h). Endoperidial surface under SEM (K(M)138625, epitype), bar = 100 μm.
Fig 6.
Dickson’s illustration of Myriostoma coliforme (lectotype) published in 1785 as (Tab. III: Fig 4).
Fig 7.
Distribution map of the Myriostoma specimens included in the phylogenetic analyses of this study (geometric figures in colour).
This figure was made using the software Quantum Gis 2.8 (QGIS), a free and open source geographic information system (https://www.qgis.org/es/site/about/index.html). The dark grey areas correspond to the distribution of Myriostoma coliforme s.l. according to http://iucn.ekoo.se/iucn/species_view/122233/ (accessed on 12.01.2017) and Fraiture & Otto [22].