Table 1.
Cytogenetic data of Camponotus (Myrmothrix).
Information connecting species; sample sites; geographic coordinates; biome and diploid chromosome number. Karyotype information: W (wild, 2n = 40), I (inversion, 2n = 40), T (translocation, 2n = 39). Cytogenetic techniques information: BC (C-banding), FL (fluorochromes CMA3/DAPI), NOR (NOR banding), FISH (Fluorescence In Situ Hybridization). Locality details: U—Uruguay; Brazillian States: MG—Minas Gerais, RJ—Rio de Janeiro, MT—Mato Grosso, AP—Amapá, PR—Paraná.
Fig 1.
Karyotypes of Camponotus (Myrmothrix) and its localities.
a) C. atriceps from Lavras; b) C. cingulatus from Viçosa; c) C. cingulatus from Rio de Janeiro; d) C. renggeri from Macapá; e) C. renggeri from Nova Mutum; f) C. rufipes from Lavras; g) C. rufipes from Viçosa; h) C. rufipes from Rio de Janeiro; i) C. rufipes from Petrópolis; j) C. rufipes from Ponte Nova; k) C. rufipes from Ubá; l) C. rufipes from Curitiba; m) C. rufipes from Urucânia. Bar = 5μm.
Fig 2.
Chromosome rearrangements of Camponotus (Myrmothrix).
a) C. renggeri with heterozygous chromosome inversion from Nova Mutum (2n = 40); b) C. renggeri with homozygous chromosome inversion from Nova Mutum (2n = 40); c) C. rufipes with heterozygous chromosome translocation from Lavras (2n = 39); d) C. rufipes with heterozygous chromosome translocation from Ponte Nova (2n = 39); e) C. rufipes with heterozygous chromosome inversion from Ponte Nova (2n = 40); f) C. rufipes with heterozygous chromosome translocation from Ubá (2n = 39); g) C. rufipes with heterozygous chromosome translocation from Viçosa (2n = 39); h) C. rufipes with heterozygous chromosome translocation from Curitiba (2n = 39). ✳—Rearranged chromosomes. Bar = 5μm.
Fig 3.
Haploid karyotypes from males of Camponotus (Myrmothrix) and its localities.
a) C. cingulatus from Rio de Janeiro; b) C. renggeri from Macapá; c) C. rufipes from Rio de Janeiro; d) C. rufipes from Ubá; e) C. rufipes from Curitiba; f) C. rufipes with heterozygous translocation from Viçosa (n = 19). The box points a chromosome result of a translocation. Bar = 5μm.
Fig 4.
C-Banded metaphases of Camponotus (Myrmothrix).
a) C. atriceps from Lavras; b) C. cingulatus from Rio de Janeiro (haploid male); c) C. renggeri from Nova Mutum; d) C. rufipes from Lavras. Bar = 5μm.
Fig 5.
Fluorochrome treated metaphases of Camponotus (Myrmothrix).
(a, b) Camponotus rufipes; (c, d) C. renggeri; (e, f) C. atriceps; (g, h) C. cingulatus stained with DAPI and CMA3 respectively. Arrows point GC-rich/AT-poor regions. Bar = 5μm.
Fig 6.
Fluorochrome treated metaphases of Camponotus (Myrmothrix) with chromosome rearrangements.
All images are stained respectively with DAPI and CMA3. (a, b) C. renggeri with homozygous inversion (2n = 40); (c, d) C. rufipes with heterozygous translocation or hybridism (2n = 40); (e, f) C. renggeri with heterozygous inversion (2n = 40); (g, h) C. rufipes with heterozygous translocation (2n = 40). Arrows point GC-rich/AT-poor regions. Arrowheads point the rearranged chromosome. Bar = 5μm.
Fig 7.
Camponotus (Myrmothrix) metaphases submitted to the FISH technique for detecting 18S and 5S rDNA clusters.
(a, b) C. cingulatus from Viçosa; (c, d) C. renggeri from Nova Mutum; (e, f) C. rufipes from Viçosa showing 18S rDNA and 5S rDNA respectively; g) C. renggeri from Macapá showing 18S rDNA clusters; h) C. rufipes with heterozygous chromosome translocation (2n = 39) from Viçosa showing 18S rDNA clusters. Bar = 5μm.
Fig 8.
NOR banding metaphases of Camponotus (Myrmothrix).
a) C. renggeri from Nova Mutum; b) C. rufipes from Lavras. Bar = 5μm.