Fig 1.
Mutational landscape of TCL cell lines.
The results of targeted deep sequencing of 16 genes in 20 T-ALL (black), 5 ALCL (dark grey), 3 CTCL (medium grey), 2 NK (light grey), 2 ATLL (diagonal lines) and one T-LGL (dots) cell lines. Mutated genes (rows) are arranged in decreasing order of mutation frequency. Cell lines (columns) are arranged from left to right on the basis of their mutational frequency following gene ranking. HTLV-1-positive cell lines (green) and translocation t(2;5)(p23;q35) (ALK +, dark blue) are showed.
Fig 2.
Mapping of variants in a TCL gene panel.
Schematic of the alterations encoded by SNVs in TP53, NOTCH1, DNMT3A, JAK1, JAK3, STAT3 and STAT5B. Type of variation and disease are represented by color and shape, respectively. TAD: transactivation domain; PRD: proline-rich domain; TD: tetramerization domain; C-term: C-terminal domain; HD: heterodimerization domain; TM: transmembrane domain; RAM: Rbp-associated molecule domain; ANK: ankyrin domain; PEST: proline (P), glutamic acid (E), serine (S), threonine (T) degradation domain; ZNF: zinc-finger domain; Mtase: methyltransferase domain.
Fig 3.
Unsupervised hierarchical clustering analysis with 26 immunomarkers.
Each row represents a single cell line; each column represents a single immunomarker. Blue (score 0); white, weak immunostaining (score 1); light red (score 2); red, strong immunoreactivity (score 3); grey, missing data.