Table 1.
Primer and probe sequences of the real time PCR panels.
Table 2.
Comparison of real time PCR results with conventional PCR results on 120 ETEC isolates and 74 E. coli pools.
Fig 1.
Correlation of Cqs between enterotoxin and CFs on cultures (A) and stool (B).
(A) On culture (N = 194), the overall correlation R2 = 0.78 (P < 0.001). Each symbol represents one CF type. (B) On stool, the Cq correlation between enterotoxin and primary/sole CF (the most abundant CF type measured by Cq values), secondary CF (the second most abundant, if present), tertiary CF (the third most abundant, if present), and quaternary CF (the fourth abundant, if present) CF were all statistically significant (P < 0.05, with R2 = 0.66 (N = 81), 0.45 (N = 39), 0.46 (N = 17), and 0.82 (N = 7), respectively. The dotted line shows the diagonal line.
Fig 2.
Comparison of CF detection on randomly selected stool samples and the corresponding E. coli pools.