Table 1.
Association between Romo1 and clinicopathologic parameters.
Fig 1.
Representative tissue samples showing different levels of Romo1 expression.
Romo1 was detected in the cytoplasm of cancer cells. (A) Romo1 expression level of 0. Magnification, x40 and x100; (B) Romo1 expression level of 12. Magnification, x40 and x100; (C) Romo1 expression level of 30. Magnification, x40 and x100.
Table 2.
The proportions of patients with low and high ROMO1 expression according to clinicopathologic parameters.
Table 3.
Survival analyses for patients who underwent curative resection.
Fig 2.
The cumulative disease-free (A) and overall (B) survival rates among patients who had curative resection by Romo1 expression levels. P values were defined by the log rank test.
Fig 3.
The cumulative overall survival rates of the whole cohort by Romo1 expression levels.
P value was defined by the log rank test.
Table 4.
Survival analyses for the whole cohort.
Fig 4.
(A) Western blotting was performed to confirm knockdown of Romo1. (B) The cell viability of the colorectal cancer (CRC) cells was determined by MTT assay after transfection with Romo1 siRNA, Romo1 shRNA, control siRNA, or control shRNA. Romo1 had no effect on cell viability and proliferation in CRC cells. (C) The effects of Romo1 on cell motility in CRC cells were determined by wound healing assay. Cell monolayers were scratched with a pipette tip and incubated with 5% FBS medium. Cell migration to the wound area was then monitored for 0hr, 24hr, and 48h post-wound, and the percentage of total area covered by the cells was then assessed using the NIH Image program. (D) Matrigel invasion assay was performed to determine the effects of Romo1 on the invasive ability of CRC cells. The invaded cells on the bottom chamber were stained with crystal violet and counted. Images of invasive HCT116 and DLD-1 cells are shown.
Fig 5.
(A) Western blotting was performed to confirm upregulation of Romo1. (B) The cell viability of the colorectal cancer (CRC) cells was determined by MTT assay after transfection with pFlag-c1 or pFlag-c1 Romo1. Romo1 had no effect on cell viability and proliferation in CRC cells. (C) The effects of Romo1 on cell motility in CRC cells were determined by wound healing assay. Cell monolayers were scratched with a pipette tip and incubated with 5% FBS medium. Cell migration to the wound area was then monitored for 0hr, 24hr, and 48h post-wound, and the percentage of total area covered by the cells was then assessed using the NIH Image program. (D) Matrigel invasion assay was performed to determine the effects of Romo1 on the invasive ability of CRC cells. The invaded cells on the bottom chamber were stained with crystal violet and counted. Images of invasive HCT116 and DLD-1 cells are shown.