Fig 1.
OTU overlap among technical replicates for experiment I.
(A) Between two technical replicates, singletons not removed; (B) between two technical replicates, singletons removed; (C) among three technical replicates, singletons not removed; (D) among three technical replicates, singletons removed.
Fig 2.
Overlap of OTUs generated using Uclust between/among technical replicates at different sequencing depth.
At a sequencing depth of about 30,000 reads, overlap of both two and three replicates was approaching a plateau when singletons were not removed.
Table 1.
Three-way ANOVA a to assess alpha diversities b at different levels for experiment I.
Table 2.
One-way ANOVA and Duncan grouping to assess β-diversity at different levels based on OTUs from Experiment I.
Fig 3.
Detrended correspondance analysis of the microbial communities from Experiment I, with singletons not removed.
Samples were from three experiment locations (H, Hailun; F, Fengqiu; Y, Yingtan) with two treatments at each location: planted (P) and unplanted (C, control), and three field replicates for each treatment. Each soil was tagged three times to create technical replicates. The three technical replicates of each soil were sequenced in the same MiSeq run. Singletons were not removed.