Table 1.
Primers used for amplification of HSVgB, gC, gD, and gE genes.
Table 2.
Number of subjects (isolates).
Fig 1.
gD amino acid sequences of isolates from the Herpevac Trial.
The US6 gene of isolates from subjects infected during the Herpevac Trial was sequenced and then translated. A) Diagram of the gD protein showing boundaries of functional domains; TM, transmembrane. 309t in red indicates the site of truncation in the HSV-2 gD vaccine construct. Substitutions in the gD amino acid sequences of isolates from B) 39 HSV-1-infected subjects compared with reference sequence KOS (HSV-1 laboratory strain), and C) 44 HSV-2-infected subjects compared with G (HSV-2 laboratory strain and derivation of the gD-2 vaccine), and SD90e (HSV-2 primary isolate from South Africa) are shown. Numbers to the right of each sequence representation indicate the number of subjects sharing the amino acid sequence. C, control vaccinated subject; V, gD-2 vaccinated subject.
Fig 2.
gC amino acid sequences of isolates from the Herpevac Trial.
The UL44 gene encoding gC was sequenced from selected subjects’ isolates and aligned to reference sequences, then translated. Amino acid substitutions are diagramed for A) gC from HSV-1-infected subjects, B) gC from HSV-2-infected subjects. Numbers to the right of each sequence representation indicate the number of subjects sharing the amino acid sequence. C, control vaccinated subject; V, gD-2 vaccinated subject. The triangle represents a deletion of one amino acid.
Fig 3.
gE amino acid sequences of isolates from the Herpevac Trial.
The US8 gene encoding gE was sequenced from selected subjects’ isolates and aligned to reference sequences, then translated. Amino acid substitutions are diagramed for A) gE from HSV-1-infected subjects, and B) gE from HSV-2-infected subjects from the same sets of subjects as described in Fig 2. Numbers to the right of each sequence representation indicate the number of subjects sharing the amino acid sequence. C, control vaccinated subject; V, gD-2 vaccinated subject. The inverted triangle represents an insertion of two amino acids (GE).
Fig 4.
The UL27 gene encoding gB was sequenced from selected subjects’ isolates and aligned to reference sequences. Amino acid substitutions in the translated sequence are diagramed for gB from A) HSV-1-infected subjects, and B) HSV-2-infected subjects from the same sets of subjects as described in Fig 2. Numbers to the right of each sequence representation indicate the number of subjects sharing the amino acid sequence. C, control vaccinated subject; V, gD-2 vaccinated subject.
Table 3.
Nucleotide sequence diversity in glycoproteins B, C, D and E among HSV-1 and HSV-2 primary isolates.
Fig 5.
dN/dS ratios for HSV-1 and HSV-2 glycoprotein gene sequences.
Mean ratio of non-synonymous to synonymous evolutionary substitutions (dN/dS) within the US27 (gB), UL44 (gC), US6 (gD) and US8 (gE) genes is shown for all HSV-1 and -2 strains sampled. The dN/dS ratio for all HSV-2 gD sequences from gD-2 vaccine recipients was not significantly different than control subjects (0.61 versus 0.53, P = 0.554 by unpaired t test).