Fig 1.
The four regions used for considering the sympatry of sister OTUs (A), and map of the Indo-West Pacific region indicating the sampling sites for the taxa examined in the present study (B). Abbreviations: IO, Indian Ocean; ECS, East China Sea; SCS, South China Sea; CT, Coral Triangle region; SAS, Sahul Shelf and Australia. Dots in different color represent the examined samples from different sources. Red dots: our own collections; green dots: online databases.
Fig 2.
Summarized phylogenetic tree of the IWP sciaenids inferred by partitioned maximum-likelihood method with GTR+Γ+I nucleotide substitution model based on the COI gene dataset and results from species delimitation analyses and other considerations by four additional criteria (see materials and methods).
Branch lengths are proportional to the inferred nucleotide substitutions. Numbers at nodes represent bootstrap values in percentage. Values below 70% are not shown; * indicates 100%. Taxa name in bold indicates problematic or probable misidentification samples. Numbers within the parentheses shown after the taxon names indicate number of sequences being contained within each collapsed clade or lineage. The tree is rooted with Aplodinotus grunniens according to Lo et al. [24]. Summary of the determined OTUs (vertical bars) is presented on the right side of the phylogenetic tree. Black bar indicates congruent OTUs as suggested by both species delimitation methods (i.e. robust results). White bar with a cross inside indicates the presence of multiple OTUs determined by GMYC in the particular genetic lineage that is incongruent to ABGD result. Missing data are marked by black crosses. Abbreviations for geographic distribution of the taxa: IO, Indian Ocean; ECS, East China Sea; SCS, South China Sea; CT, Coral Triangle region; SAS, Sahul Shelf and Australia.
Fig 3.
Phylogenetic tree of the IWP sciaenids inferred by partitioned maximum-likelihood method with GTR+Γ+I nucleotide substitution model based on the combined dataset.
Branch lengths are proportional to the inferred nucleotide substitutions. Numbers at nodes represent bootstrap values in percentage. Values below 70% are not shown; * indicates 100%. Different colors in genus Johnius show that the taxa are either from the pre-recognized subgenus, Johnius (in blue) or subgenus Johnieops (in red). The tree is rooted with Aplodinotus grunniens according the result shown in Lo et al. [24]. Abbreviations for geographic distribution of the taxa: IO, Indian Ocean; ECS, East China Sea; SCS, South China Sea; CT, Coral Triangle region; SAS, Sahul Shelf and Australia.