Fig 1.
Stations Sampled for eDNA metabarcoding analysis.
All are located within the Monterey Bay National Marine Sanctuary. See Table 1 for bottom depth of each station; green stations are on the shelf (<200 m water column depth) and blue stations are in a canyon (>200 m water column depth). Isobaths are labeled with water column depth in meters.
Table 1.
Sample collection metadata.
Table 2.
Number of sequencing reads retained during data processing.
Fig 2.
Percent of OTUs identified in 1, 2 or 3 of the biological replicates collected at each station/sampling depth.
Samples are labeled with station (i.e., 1F, 2F, etc.) followed by the sampling depth (i.e., 0 m, 20 m, 40 m). 3F-0 m and 5F-0 m are not shown because they do not have complete sets of three replicates after rarefying.
Table 3.
Taxa identified using edna metabarcoding.
Table 4.
Presence/Absence of 33 families at each station identified during the study across biological replicates and sampling depths.
Fig 3.
Families identified using eDNA metabarcoding in samples collected at stations on the continental shelf (water column depth < 200 m) and stations in a canyon (water column depth >200 m).
20 families were only found on the shelf, 2 were only found in a canyon, and 11 were found at both.
Fig 4.
Families identified using eDNA metabarcoding in samples collected at surface and at subsurface (20/40 m).
6 families were only found when sampling at the surface of the water, 6 families were only found when sampling the subsurface (20/40 m), and 21 of 33 families were found both at surface and subsurface.