Table 1.
List of marker used for blast resistance genes.
Fig 1.
Reaction of rice genotypes to leaf blast in uniform blast nursery (UBN) at Cuttack.
Table 2.
Evaluation of NRRI released varieties (NRVs) for leaf blast resistance in the uniform blast nursery.
Fig 2.
Agarose gel photograph of 22 NRVs, to know the presence or absence of A) 388 bp of Pib rice blast resistance gene amplified with SNP marker Pb28; B) 292 bp of Piz rice blast resistance gene amplified with SNP marker z56592; C) 2000 bp of Pi9 rice blast resistance gene amplified with gene based STS marker; D) 1500 bp of Pik-h rice blast resistance gene amplified with gene based marker. M denotes ladder; lane 1 positive control; lane 2 CO39 (negative control); lanes 3–24: rice varieties.
Fig 3.
Reaction of rice genotypes to leaf blast in uniform blast nursery (UBN) at Cuttack and number of R genes they possessed.
Fig 4.
Clustered analysis of 80 NRVs based on R genes.
Table 3.
Association of rice blast resistant genes with blast disease in 80 NRVs.
Fig 5.
Population structure of 80 NRVs based on 17 markers (K = 2) and graph of estimated membership fraction for K = 2.
The maximum of adhoc measure ΔK determined by structure harvester was found to be K = 2, which indicated that the entire population can be grouped into two subgroups. Different color within group indicates the proportion of shared ancestry with other group which has the same color with the admixture.
Fig 6.
Two dimensional PCoA display of 80 NRVs based on 17 markers. Coord 1 and Coord 2 represent first and second coordinates, respectively.
The two PCA axes accounted for 27.55% and 18.40% of the genetic variation among populations.
Table 4.
Results of analysis of molecular variance (AMOVA).
Table 5.
Pair-wise FST estimates among the three populations of NRVs.
Table 6.
Durability of resistant NRVs against leaf blast.