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Fig 1.

Sampling stations of the RoME Project.

The black dots indicate the sampling stations. The bathymetry data of the Ross Sea from [43] under a CC BY license, with permission from [Davey], original copyright [2004–2005].

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Fig 2.

Sampling stations within each area, corresponding map of the upper mixed layer (UML) and the Θ/S diagram.

Station maps for the RoME 1, 2 and 3 (A, D, G, respectively). The bathymetry data of the Ross Sea from [43] under a CC BY license, with permission from [Davey], original copyright [2004–2005]. Map of the UML depth (colour scale) for the three areas (B, E, H). The Θ/S diagrams for each leg with indication of data shallower than the UML (red dots) and of the freezing point (C, F, I). UML maps and Θ/S diagrams produced through Ocean Data View software [44].

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Fig 3.

Upper mixed layer (UML) depth (m) in the three areas (boxplot by RoME).

The red line shows the median, and the red cross the mean.

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Table 1.

Mean (±standard deviations), minimum and maximum values, calculated in the upper mixed layer (UML) (A) and below the UML (B) for the three sampled areas.

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Fig 4.

Environmental features in the two investigated layers of each area.

Temperature (°C), melt-water (%) and DIN [dissolved inorganic nitrogen] concentrations (μM). The red cross shows the mean. Samples in the UML (A) and samples below the UML (B).

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Fig 5.

Biological features in the two investigated layers of each area.

Total biomass (mg Chl-a m-3), micro (>20 μm) fractions (%), diatoms (%), Haptophytes (%) and Fv/Fm [maximum quantum yield]. The red line is the median and red cross is the mean. Samples in the UML (A) and samples below the ULM (B).

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Fig 6.

Results of PCA applied to physical, chemical and biological parameters.

Parameters included in the analysis: ΔSi/Fuco [ΔSi(OH)4/Fucoxanthin], Haptophytes (%), Si(OH)4 [silicate], DIN [dissolved inorganic nitrogen (NO3- + NO2- + NH4+)], PO43- [phosphate], Fv/Fm [maximum quantum yield], Total Chl-a, Diatoms (%), MW [melt-water] (%) and Temperature (°C). The samples in the UML (full shot-A) and samples below the ULM (cross-B): RoME 1, blu; RoME 2, green; and RoME 3, red.

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Table 2.

Correlation matrix (Spearman) among physical, chemical and biological variables.

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Table 2 Expand

Table 3.

Spearman correlation matrixes between the phytoplankton and the POM features (quantitative: POC, protein and carbohydrate concentrations; qualitative: Protein to carbohydrate ratio, and POC/PON ratio) for the three sampled areas.

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