Fig 1.
Protein coronas, formed around 76 nm silica particles in whole blood and different blood derivatives.
Top panel: protein corona formed in (1) whole blood (2) EDTA stabilized whole blood (3) EDTA stabilized blood plasma and (4) blood serum. The respective blood fractions are shown in the control lanes (1c, 2c, 3c and 4c) The * marks the lane with Mw standards with Mw in kDa. Bottom panel: comparison of the intensity of gel bands after band intensity analysis [41, 42] for coronas and controls. The arrows high-light some of the differences found between different blood derivatives.
Fig 2.
Protein coronas, formed around 9.5 nm silica particles in different blood derivatives.
Top panel: 1 = protein corona from whole blood, 2 EDTA stabilized whole blood, 3 blood plasma, and 4 blood serum. The * marks the Mw standard.
Fig 3.
Comparing the intensity of different bands for two plasma corona from silica nanoparticles.
Plasma corona from 9.5 nm silica particles in green and from 76 nm silica particles in blue. The x-axis has been adjusted for the 10 nm silica sample since the data comes from different gels.
Fig 4.
Protein corona SDS-gel bands analyzed with mass spectrometry.
Lanes a and b: the plasma protein corona around 76 nm silica particles. Lane c: the plasma protein corona formed around 9.5 nm silica particles. Arrows indicate areas that were excised for analysis by limited proteolysis and the results are listed in Table 1. * is the molecule weight standard.
Table 1.
Identified proteins.
Fig 5.
Nanoparticle induced thrombin generation.
Black line) 9.5 nm particles, dashed black line) 13 nm particles, gray line) 23 nm particles, dashed gray line) 76 nm particles and dotted black line) control. The first derivative, fluorescence units/min, is shown (means of n = 3).