Fig 1.
Schematic description of denaturation of proteins by surfactant.
Fig 2.
The diffusion coefficient for the β-lactoglobulin-SDS (A), transferrin-SDS (B) and human insulin-SDS (C) complexes as a function of SDS concentration determined using the Taylor dispersion analysis.
Fig 3.
ECD spectra showing changes in the tertiary structure of β-lactoglobulin (A), transferrin (B) and human insulin (C) with increasing surfactant concentration. For β-lactoglobulin and transferrin the concentrations of SDS were 4.3 x 10−4 M and 8.7 x 10−2 M for partially and fully denatured protein, respectively. For insulin the concentrations of SDS were 2.3 x 10−4 M and 8.7 x 10−2 M for partially and fully denatured protein, respectively.
Table 1.
The ratio of the number of micelles to the number of proteins.
Table 2.
Comparison of diffusion coefficients determined using the Taylor dispersion analysis and dynamic light scattering before and after addition of SDS at high concentration to the solution.