Fig 1.
The principle of an activity integrated strategy method.
The UPLC system with auto-fraction was used to separate, quantify collect and enrich the active compounds in fraction were performed by an. The fractions were used to test the bioactive analysis.
Table 1.
The information of the 18 batches of Radix scutellariae.
Fig 2.
(A) RS extracts, (B) 9 standards mixture. Scutellarin (5), scutellarein (10), baicalin (11), chrysin-7-O-glucuronide (15), baicalein (19), wogonoside (22), wogonin (23), chrysin (24) and oroxylin A (25).
Table 2.
UHPLC data for the calibration curves, LOQs, LODs and repeatability (n = 6).
Table 3.
Intra-day, inter-day precision and stability of the seven bioactive compounds (n = 6).
Table 4.
The recovery of the eight bioactive compounds (n = 3).
Fig 3.
Chromatogram of activity-integrated fingerprints of RS extracts.
Chromatography (A) and total ion chromatography (B) of 26 compounds with NA inhibitory activity. Colored bar means the fraction collection purity > 98%.
Table 5.
The qualitative information of Radix scutellariae.
Fig 4.
The NA inhibitory activity of compounds isolated from RS.
The IC50 of Radix scutellariae extract scutellarin, scutellarein, baicalin, baicalein, wogonoside, wogonin, chrysin-7-O-glucuronideand chrysin by an NA inhibitory screening kit.
Fig 5.
The cellular toxicity of baicalin, baicalein, wogonoside and wogonin.
PLF and HIEC-6 cells were treated with indicated drugs with different concentrations as indicated for 24 hours and collected to evaluate cellular viability.
Fig 6.
The relative NA inhibitory activity of baicalin, wogonin and wogonoside on HEK293Tcells.
Table 6.
The quantitative results of Radix scutellariae.
Fig 7.
HCA dendrogram of 18 RS samples based on the NA inhibitory activity of compounds.
Biennially cultivated RS (Group 1), perennially cultivated RS (Group 2), wild RS (Group 3) and plateau area RS (Group 4).