Fig 1.
Root tips of Pinus armandii in association with Tuber indicum (a and b) or without T. indicum (c and d).
Table 1.
Community richness and diversity indices of bacteria associated with Pinus armandii roots and surrounding soils with or without Tuber indicum partner.
Table 2.
Community richness and diversity indices of fungi associated with Pinus armandii roots and surrounding soils with or without Tuber indicum partner.
Table 3.
Physical and chemical properties of Pinus armandii rhizosphere and ectomycorrhizosphere soil.
Fig 2.
Hierarchical cluster analysis of the similarities among different samples based on the soil properties using the the between-groups linkage method in SPSS.
CK.S, rhizosphere soil; ECM.S, ectomycorrhizosphere soil.
Fig 3.
Taxonomic composition of bacterial and fungal communities associated with Pinus armandii root tips and surrounding soils at the phylum and class levels.
ECM and ECM.S, ectomycorrhizae (Pinus armandii mycorrhized with Tuber indicum) and ectomycorrhizosphere soil. CK and CK.S, roots and soils from cultivated P. armandii without T. indicum partner. a, bacterial phyla; b, bacterial classes; c, fungal phyla; d, fungal classes. All experiments were conducted in triplicate.
Fig 4.
Heat-map analysis of the 35 most abundant bacterial and fungal genera in Pinus armandii roots and surrounding soils.
ECM and ECM.S, ectomycorrhizae (Pinus armandii in association with Tuber indicum) and ectomycorrhizosphere soil. CK and CK.S, roots and soils from cultivated P. armandii without T. indicum partner. a, bacterial genera; b, fungal genera. The relative abundance of the sample at genus level increased with the increase of the color block value.
Fig 5.
Principal Coordinate Analysis (PCoA) of bacterial (a) and fungal (b) communities associated with Pinus armandii roots and surrounding soils with or without Tuber indicum partner based on weighted UniFrac analysis.
ECM and ECM.S, ectomycorrhizae (Pinus armandii mycorrhized with Tuber indicum) and ectomycorrhizosphere soil. CK and CK.S, roots and soils from cultivated P. armandii without T. indicum partner.