Fig 1.
Expression pattern of anterior-localized genes in the limb bud based on EMBRYS.
Candidate transcription-associated factors involved in limb bud patterning were selected from EMBRYS and classified according to their anterior expression patterns. (A: Anterior; P: Posterior).
Fig 2.
Expression pattern of candidate’s specifying the central part of the mouse limb bud based on EMBRYS.
Candidate transcription-associated factors involved in limb bud patterning were selected from EMBRYS and classified according to their expression pattern, particularly those showing significant localization in the limb bud mesenchyme. (A: Anterior; P: Posterior).
Fig 3.
Expression pattern of candidates that are anterior regulators but expanded posteriorly in Shh-KO mouse limb buds.
Whole-mount in situ analysis was performed upon limbs of Shh-KO embryos (E10.5–11.5). Disruption of Shh resulted in posteriorly expanded expression of candidates in the developing limb bud (Control: WT or Shh +/-; FL: Forelimb; HL: Hind limb; A: Anterior; P: Posterior).
Fig 4.
Reduced expression of Irx3 in Gli3-deficient limb buds.
Whole-mount in situ analysis of Gli3xt/xt embryos (E10.5–12.5). Expression of Irx3 was reduced while no significant change was seen in the expression of Asb4 and Lhx2 in Gli3-deficient limb buds. (Control: WT or Shh +/-; FL: Forelimb; HL: Hindlimb; A: Anterior; P: Posterior).
Fig 5.
Generation and phenotypic analysis of transgenic mice (Prrx1-Irx3) overexpressing Irx3 in the developing mouse limb bud.
(A) Generation of transgenic mice overexpressing Irx3 in the developing limb bud. (B) Eight fold enhanced expression of Irx3 in the limb tissue of Prrx1-Irx3 than the control (Irx3-Tg/Cre -). All data were expressed as the means ± SEM, (n = 5). *P < 0.05. (C-D) Different phenotypes of Prrx1-Irx3 autopod were classified according to (C) apparent or (D) skeletal malformation (Prrx1-Irx3: Irx3-Tg and Cre-Tg; Ctrl: Irx3-Tg and Cre (-)).