Fig 1.
The effect of apigenin (A) and TNFα (B) on cell viability of MDA-MB-231 cells at 5% CO2/Atm for 24 Hr. The data are presented as viability (% Ctrl), Mean ± S.E.M. n = 4. The significance of differences from the Ctrl and were determined by a one-way ANOVA, with a Tukey post hoc test. *p<0.05.
Fig 2.
(A-D). The release of cytokines as assessed using Human Cytokine Array C1000 membranes from MDA-MB-231 cells ± TNFα ± Apigenin. [A] Ctrls, [B] TNFα-treated (40ng/mL) [C] Apigenin (40μM) and [D] TNFα-treated (40ng/mL) + Apigenin (40μM) co-treated cells.
Fig 3.
(A-D). The release of cytokines as assessed using Human Cytokine Array C1000 membranes from MDA-MB-231 cells ± TNFα ± Apigenin. [A]Ctrls, [B]TNFα-treated (40ng/mL) [C]Apigenin (40μM) and [D]TNFα-treated (40ng/mL) + Apigenin (40μM) co-treated cells. The data present membrane spot densities (top) and manufacturer’s grid layout (bottom).
Fig 4.
The effect of apigenin on TNFα mediated changes in GM-CSF (A) IL-6 (B), CCL2 and IL-1α (C) in MDA-MB-231 cells. The data represent the densitometry values (expressed as intensity per square millimeter) (INT/MM2) per spot as Mean ± S.E.M. n = 3. The significance of differences between the Ctrl and TNFα groups and TNFα vs. TNFα + Apigenin were determined by a Students t-test *p<0.05.
Fig 5.
Effect of Apigenin on TNFα mediated changes in CCL2 (A) and IL-1α (B) in MDA-MB-231 cells. The data represent CCL (pg/ul) or IL-1α (O.D. @ 405nm) as Mean ± S.E.M. n = 3. The significance of differences between the Ctrl and TNFα groups and TNFα vs. TNFα + Apigenin were determined by a Students t-test *p<0.05.
Fig 6.
NF-kB PCR microarray assessment on MDA-MB-231 cells ± TNFα ± Apigenin.
The data displays differential transcription for controls vs. TNFα (A) and TNFα vs. TNFα + Apigenin (B) in MDA-MB-231 cells treated for 24 hours. The data show no significant changes at P < .05 for either analysis, with non-significant differences for only IL-1α.
Fig 7.
A. IKBKe transcription in MDA-MB-231 cells ± TNFα ± Apigenin. The data represent normalized expression and are expressed as the Mean ± S.E.M., n = 3. The significance of differences between the Ctrl and TNFα groups and TNFα vs. TNFα + Apigenin were determined by a Students t-test *p<0.05. Figure 7B. Protein expression of IKBKe/GAPDH with TNFα ± Apigenin at 24 hours.
Fig 8.
Protein expression of ERK 1 2 (total and phosphorylated) and GAPDH in MDA-MB-231 cells ± TNFα ± Apigenin.
Table 1.
NF-kB PCR microarray tabulated data on MDA-MB-231 cells ± TNFα ± Apigenin.
The data displays differential transcription as Log2 (Fc) for controls vs. TNFα (Left Panel) and TNFα vs. TNFα + Apigenin (Right Panel) in MDA-MB-231 cells treated for 24 hours. The data show no significant changes at P < .05 for either analysis, with non-significant differences for only IL-1α.