Table 1.
Loci targeted for PCR using sourced primers.
Forward (-F) and reverse (-R) primer directions indicated by suffix. Original primer sequences modified by addition of 17 bp vector M-13 5´ tails (tail sequences not shown here, refer to Materials and methods).
Fig 1.
Maximum percent intraspecific distance (Dintra), and percent distance to nearest genetic neighbour species for six loci across five invasive and seven native grass species.
A: atpF; B: psbE; C: matK; D: ETS; E: ndhK; F: ITS.
Table 2.
PCR success rate of each locus against each species tested (Invasive grasses are indicated with *).
Table 3.
Information used to evaluate the utility of the six loci.
Table 4.
Percent distance of maximum percent intraspecific distance (Dintra) and nearest genetic neighbour species (DNN) across five invasive and seven native grass species (N/A: not available for single specimen).
Fig 2.
NJ trees inferred from six loci.
A: atpF, B: psbE, C: matK, D: ETS, E: ndhK, F: ITS. Bootstrap support > 70% (N = 1000 replications) for clusters as reported. Scale bars indicate proportion of differences under a K2P model.
Fig 3.
ML trees inferred from six loci.
A: atpF, B: psbE, C: matK, D: ETS, E: ndhK, F: ITS. Bootstrap supports > 70% (N = 1000 replications) for clades as reported. Scale bars report proportion of differences under a GTR model.
Table 5.
Overview of the multiple loci combinations analyses.