Fig 1.
Evaluation of CD68, CD204 and CD206 expression in cultured THP-1-derived macrophages.
Immunocytochemistry of CD68 (marker of macrophage activation), CD204 and CD206 protein expression in cultured THP-1-derived macrophages (M0 macrophages) treated for 72 hours with ET-1 (100nM) and IL-4 (10ng/mL) alone, or pre-treated with ETA/BRA (bosentan, 10-5M) for 1 hour before being stimulated with ET-1. Cultured M0 macrophages maintained for 72 hours in RPMI at 5% of FBS were used as controls (M0-controls). Immunocytochemistry was performed on four independent in vitro experiments. The detection of protein expression was performed evaluating the same number of cells by light microscopy (magnification 40X).