Table 1.
Requirements and technical solution applied in the new intermittent hypoxia system.
Fig 1.
A block diagram of the complete setup (A), the speed and pattern of lowering the oxygen to a set concentration (B), a block diagram detailing the controller and the dual sensor interface (C). The solid line denotes airflow and the dashed line the electrical connections. FPGA, field programmable gate array; DC-DC digital to digital converter.
Fig 2.
A photograph of how the mouse cage lid was modified to fit the regulating apparatus of the system.
Fig 3.
Representative images of neuronal apoptosis in the hippocampal region cornu ammonis 3 (CA3) of mouse from the control group (A) and intermittent hypoxia group (B). The arrows show apoptotic neurons.
Fig 4.
Apoptotic neurons per mm3 of tissue in the control group (CTRL) compared to the intermittent hypoxia group (IH) in cornu ammonis 1(CA1) subfield (P< 0.001) (A), cornu ammonis 3 (CA3) subfield (P = 0.001) (B), and the dentate gyrus (DG) (P = 0.023) (C) shown as columns representing means and standard deviations. The number of apoptotic neurons differed significantly in the DG IH group opposed to CA1 IH (P<0,001), and CA3 IH (P<0,001), as well as in the DG CTRL group opposed to CA1 CTRL (P<0,001), and CA3 CTRL (P<0,001) (D). Asterisks denote statistically significant differences.