Fig 1.
Circular map of the P. aeruginosa PA1 genome.
The outermost concentric ring shown coding sequences (CDS) in blue on the plus (outer) and minus (inner) strands. tRNA genes are shown in light red and rRNA genes in light purple. Proceeding inwards, the next ring shows local GC content in black as a deviation from the average value. GC skew is shown on the next ring using green and purple, and genome coordinates are shown on the innermost ring. The predicted prophage clusters are highlighted in orange.
Table 1.
Predicted prophage clusters in P. aeruginosa strain PA1.
Fig 2.
Excision and circularization of pp3 from the PA1 genome.
(A) Schematic of pp3 excision and circularization. Primer pairs D-F/D-R and C-F/C-R are indicated. (B) Detection of pp3-excised PA1 chromosome (lanes labeled D) and circular form (lanes labeled C) of pp3. From left to right, genomic DNAs from strains PA1, PA1Δint3 and PA1Δint3::C were used as PCR templates. Lane M contains DNA size markers. (C) Sequencing of PCR products amplifying across the attB and attP sites upon pp3 excision and circularization. The 26-bp attachment site of pp3 is indicated by a red box.
Fig 3.
Genetic organization of prophage pp3.
Genes are indicated as arrows showing the direction of transcription. Gray arrows represent genes encoding hypothetical proteins.
Fig 4.
Interstrain transfer of prophage pp3 between P. aeruginosa strains.
(A) PCR analyses of strains used to test horizontal transfer of pp3 from PA1 to PAO1 (see text for detailed strain descriptions). Lane M contains a DNA size marker. (B) Confirmation of pp3 integration into the predicted attachment site in strain PAO1. The diagram on the left shows the expected PCR products. Lanes marked CF represent the circular form of pp3. Lane M contains a DNA size marker.
Fig 5.
Biofilm formation is increased by transfer of pp3.
Data are from three independent experiments and are shown as means ± standard deviations. **, P< 0.01.