Fig 1.
Gene Ontology classifications of assembled non-redundant unigenes.
Fig 2.
KEGG pathway classification of non-redundant unigenes.
A: Cellular Processes; B: Environmental Information Processing; C: Genetic Information Processing; D: Metabolism; E: Organismal Systems.
Fig 3.
Venn diagram showing differentially expressed genes in brain (A) and gonad tissue (B) from three sex stages.
F: female, I: intersex, M: male.
Fig 4.
GO classification for differentially expressed genes in gonad between stages.
A: up-regulated genes comparing F_gonad to I_gonad. B: down-regulated genes comparing F_gonad to I_gonad. C: up-regulated genes comparing I_gonad to M_gonad. D: down-regulated genes comparing I_gonad to M_gonad.
Fig 5.
GO classification for differentially expressed genes in gonad between female and male stage.
A: up-regulated genes. B: down-regulated genes.
Fig 6.
RT-qPCR validation for 9 gonad differentiation related genes.
The “*” stands for p < 0.05.
Fig 7.
In situ hybridization for foxn5 in female gonad of rice field eel.
The digoxigenin label was incorporated into the single-stranded probe, and a sense a sense probe was used as negative control. It showed that the expression of foxn5 was located in the egg envelope and the early phase of follicles. A: nucleus dyed by PI; B: localization of foxn5, marked by white arrow: C: merged A and B. D-F for negative control.
Table 1.
Partial candidate unigenes screened from gonad transcriptsm of M. albus.