Fig 1.
Flowchart showing results of bacterial multiplex realtime-PCR compared to cerebrospinal fluid microscopy and culture.
Fig 2.
Results of bacterial and viral multiplex realtime PCR assays in cases of suspected meningitis in children presenting to the acute care or outpatient departments of the Red Cross War Memorial Children’s Hospital in Cape Town, South Africa, November 2012–October 2013 (n = 292).
Fig 3.
Monthly variation in detection of selected pathogens using bacterial and viral multiplex realtime PCR assays, in cases of suspected meningitis in children presenting to the acute care or outpatient departments of the Red Cross War Memorial Children’s Hospital in Cape Town, South Africa, November 2012–October 2013 (n = 292).
Table 1.
Laboratory results of 10 cases classified as confirmed cases of bacterial meningitis according to the definitions of the World Health Organisation (WHO) Coordinated Invasive Bacterial Vaccine Preventable Diseases (IB-VPD) Surveillance Network.
Table 2.
Clinical history and CRP results of 10 cases classified as confirmed cases of bacterial meningitis according to the definitions of the World Health Organisation (WHO) Coordinated Invasive Bacterial Vaccine Preventable Diseases (IB-VPD) Surveillance Network.
Table 3.
Laboratory results of 6 cases in which bacterial target DNA was detected with bacterial multiplex realtime- PCR, but which were not confirmed according to the definitions of the World Health Organisation (WHO) Coordinated Invasive Bacterial Vaccine Preventable Diseases (IB-VPD) Surveillance Network.
Table 4.
Clinical history and CRP results of 6 cases in which bacterial target DNA was detected with bacterial multiplex realtime- PCR, but which were not confirmed according to the definitions of the World Health Organisation (WHO) Coordinated Invasive Bacterial Vaccine Preventable Diseases (IB-VPD) Surveillance Network.
Table 5.
Sensitivity and specificity of bacterial multiplex realtime-PCR for the diagnosis of bacterial meningitis compared to CSF culture and compared to laboratory-confirmed bacterial meningitis cases according to the definitions of the World Health Organisation (WHO) Coordinated Invasive Bacterial Vaccine Preventable Diseases (IB-VPD) Surveillance Network[37].
Table 6.
Analysis of multiplex RT-PCR results according to discharge diagnosis.