Fig 1.
Maximum likelihood phylogenetic tree of T. hirsuta PODs, characteristic PODs amino acid residues and genes’ intron positions.
Numbers at nodes are bootstrap percentages from 100 replicates. The outgroup containing the three peroxidase genes of Auricularia subglabra (XM_007341671.1, XM_007341680.1 and XM_007347393.1) is highlighted in pink. Characteristic LiP residues are highlighted in violet; characteristic MnP residues–in green and red. Non-standard aa residues are highlighted in blue. The numeration of aa residues is corresponding to P. chrysosporium PODs (MnP1 and LiP -H8) [37].
Table 1.
Properties of the predicted protein sequences for T. hirsuta peroxidases.
Genes with high expression rate/RQ (according to ddPCR data, discussed below) are marked in dark grey.
Fig 2.
Relative expression of PODs genes under different culture conditions. Gene wise expression of PODs.
Each doted square represents absolute expression normalized on internal control gene expression (RQ). The expression correlates both with the size of a dot and the color of a box. Samples in which expression was not detected depicted as small unsquared dots. GP, BR and AL stand for glucose-peptone medium and glucose-peptone medium with addition of bromocresol green and alkali lignin respectively.
Fig 3.
Total relative expression of PODs complex at different growth stages and under different culture conditions of T. hirsuta.
I, lag phase; II, exponential growth phase; III, stationary and lysis phase. The samples were taken in 12 h interval. Each dot represents arithmetic mean from three biological replicates on each cultivation media (GP, BR and AL). Polynomial regression line is superimposed on the mean scatter plot. Error bars represent standard deviations.