Fig 1.
Myobatrachus gouldii live, in situ, Kalbarri National Park, WA.
Photo credit: Stephen Zozaya.
Fig 2.
Haplotype network of 42 Myobatrachus gouldii samples based on the combined ND2 and RPL35 data, with mutations shown as cross bars.
The distribution of samples throughout south-western Australia are overlain onto a digital elevation model image (Shuttle Radar Topography Mission) where light grey equates to areas of high elevation, and dark grey equates to areas of low elevation. Black dashed line on the map represents the rainfall provinces after Hopper & Goia [38] (TRP: Transitional Rainfall Province, HRP: High Rainfall Province, SCP: South Coast Province), while the white dotted line refers to the location of the Darling Scarp. (image of M. gouldii from Yanchep National Park courtesy of M. Anstis).
Table 1.
Locality information for all Myobatrachus gouldii specimens sequenced in this study.
Each sample was given a laboratory identification number (Lab ID). Museum number refers to the number of the corresponding voucher specimen at the Western Australian Museum (WAM) or South Australian Museum (SAM). The locality information specifies the nearest named location as provided by the museum.
Table 2.
The names, sequences and sources of the various primers used in this study.
Primers were used for both PCR amplification and sequencing unless otherwise specified.
Table 3.
Characters comprising the morphometric data set, modified from [23].
Fig 3.
Representative calls for Arenophryne rotunda from Shark Bay, Myobatrachus gouldii from (a) Yanchep and (b) Cullacabardee north of Perth, and Metacrinia nichollsi from Albany, Western Australia. Bar represents 0.2 seconds.
Fig 4.
Plots of the first and second (A) and second and third (B) Principle Components based on analysis of the morphological data set (see text for details) to show differences between the three primary hapotype groups / geographic regions.
Fig 5.
Plots of the first and second (A) and second and third (B) Principle Components based on analysis of the morphological data set (see text for details) to show differences between the sexes.