Fig 1.
Photos of the parental accessions of the mapping populations.
(A) Spike phenotype of each parental accession. (B) Grain shape of the two subspecies.
Fig 2.
Box and dot plots for the six spikelet-related traits based on the lineages and sublineages.
Welch’s t-test was conducted for statistical significance (***P < 0.001) of differences between the two lineages, TauL1 and TauL2. Sublineages sharing a common letter were not significantly different (Steel-Dwass test, P < 0.05). The tests were performed excluding TauL3 in the interlineage analyses and TauL1x and TauL2x in the intersublineage analyses because of the lack of statistical power. Red and black dots indicate the accessions of subspecies strangulata and tauschii, respectively.
Table 1.
Eigenvectors for the first and second principal components for the six spikelet-related traits.
Fig 3.
Graph of the first three and two axes from two principal component analyses based on the six spikelet shape-related (A, B) and four grain shape-related (C) traits.
Table 2.
Grain shape variation in lineages and subspecies of Ae. tauschii.
Fig 4.
Box and dot plot comparison of grain shape between lineages and among sublineages of Ae. tauschii.
Welch’s t-test was conducted for statistical significance (***P < 0.001) of differences between the two lineages, TauL1 and TauL2. Multiplex comparison between the four sublineages was excluded because of the lack of statistical power. Red and black dots indicate the accessions of subspecies strangulata and tauschii, respectively.
Table 3.
Eigenvectors for the first and second principal components for the four grain shape traits.
Table 4.
Parental and F2 population means for eight spikelet-shape related traits in the three mapping populations.
Table 5.
Parental and F2 population means for four grain-shape related traits in the two mapping populations.
Table 6.
Summary of QTLs identified for spikelet- and grain-shape related traits in the KU-2078/PI499262 population.
Fig 5.
Comparison of the QTL positions on the chromosomes 3D (A) and 4D (B) linkage maps between the three Ae. tauschii populations.
QTLs with LOD scores above the thresholds are indicated, and genetic distances are given in centimorgans. Gray boxes indicate putative centromeric regions.
Fig 6.
Comparison of the QTL positions on the chromosome 7D linkage maps between the three Ae. tauschii populations.
QTLs with LOD scores above the thresholds are indicated, and genetic distances are given in centimorgans. Gray boxes indicate putative centromeric regions.
Table 7.
Summary of QTLs identified for spikelet- and grain-shape related traits in the KU-2003/KU-2124 and PI476874/IG47182 populations.
Fig 7.
Genotypic effects of selected QTLs on spikelet-related traits in the KU-2078/PI499262 population.
The genotypes of QTLs were inferred from genotyping data in the marker intervals shown above each graph. Means ± SD with the same letter were not significantly different (P > 0.05, Tukey-Kramer HSD test).
Fig 8.
Genotypic effects of selected QTLs on spikelet-related traits in the KU-2003/KU-2124 and PI476874/IG47182 populations.
The genotypes of QTLs were inferred from genotyping data in the marker intervals shown above each graph. Means ± SD with the same letter were not significantly different (P > 0.05, Tukey-Kramer HSD test).
Fig 9.
Phenotypic effect of the genotype combinations on spikelet morphology.