Fig 1.
Experimental definition of 14C-Met and 14C-MeAIB transport systems in HSG cells.
The uptake of 14C-Met (left) and 14C-MeAIB (right) was measured in the presence or absence of 5 mM BCH, 5 mM MeAIB, or Na+. Values are the mean ± SD.
Fig 2.
Time course of changes after irradiation by the carbon-ion beam (3-Gy) on the in vitro uptake of 14C-MeAIB (A) and 14C-Met (B) by HSG cells, and effects on the number (C) and ATP content (D) of HSG cells.
Values are the mean ± SD. Significant differences were determined by Tukey’s multiple-comparison test p<0.05 vs. non-irradiation.
Fig 3.
The uptake mechanisms of 14C-MeAIB pre- and post-irradiation with the carbon-ion beam.
The total uptake of 14C-MeAIB was divided into its transport system contributions before and after irradiation to HSG cells by the carbon-ion beam as follows: System-A, the part of uptake that is inhibited by 5 mM MeAIB. The uptake of 14C-MeAIB was measured in the presence (white bar) or absence (black bar) of 5 mM MeAIB. Values are the mean ± SD.
Fig 4.
Time course of changes in tumor volumes after irradiation by the carbon-ion beam (0 (diamond), 6 (square), or 25 Gy (triangle)).
With the 6-Gy dose, tumor volume reductions were observed and were followed by a re-growth. The irradiation of tumors with the 25-Gy dose resulted in a significant reduction in tumor size, with no tumor re-growth.
Fig 5.
Time course of changes in the uptake of 14C-Met and 14C-MeAIB in HSG tumors after irradiation by the carbon-ion beam (6- or 25-Gy).
Values are the mean ± SD. Significant differences were determined by Tukey’s multiple-comparison test p<0.05 vs. non-irradiation.
Fig 6.
In vivo uptake of 14C-Met and 14C-MeAIB at irradiation sites (solid bar) post-irradiation with the carbon-ion beam (25-Gy) and at a non-irradiated site (white bar).
Both 14C-Met and 14C-MeAIB displayed similar tissue accumulation values at the irradiated and non-irradiated muscle tissues. Values are the mean ± SD.