Fig 1.
Manhattan Plot of association P-values.
95,499 variants were investigated for association with DCM by logistic regression analysis. Associations are summarized in a Manhattan plot (R/qqman package) which displays the eleven SNVs significantly associated with DCM (Q-values < 0.01) as green dots. Note that the applied logistic model assumed an additive mode of inheritance. For variants on chromosome 15 in the ALPK3 region, a dominant mode of inheritance was better supported by the data (see Table 1 for corresponding P-values)
Fig 2.
Forest Plots of odd-ratios (log) in the different populations.
The results show that the associations were largely homogeneous across populations (See also heterogeneity column in Table 2)
Table 1.
Variants associated with DCM.
Fig 3.
Regional plots of association at 8 loci.
P-values, obtained from logistic regression analysis of genotyped and imputed variants in genomic regions demonstrating significant association with DCM are depicted. In each region, the genotyped lead-SNV is identified by its position and the other variants are colored to reflect their LD with the lead-SNV.
Fig 4.
(A) GST Pull-Down showing interaction of GFP-BAG3 expressed in HEK293 cells and recombinant GST-HSPB7. GFP-BAG3 was expressed in HEK293 cells (cell extract panel) and and GST-HSPB7 was produced in a bacterial expression system. GST-HSPB7 co-sediment with GFP-BAG3 but not with GFP alone indicating specific BAG3/GST-HSPB7 interaction (Pull-Down panel). (B) Co-immunoprecipitation experiment showing interaction of Flag-HSPB7 and GFP-BAG3 in HEK293 cells. GFP alone or GFP-BAG3 were co-expressed together with Flag-HSPB7 in HEK293 cells (cell extract panel) and subjected to immunoprecipitation with an antibody against GFP. Only GFP-BAG3 immunoprecipitated with FLAG-HSPB7 (IP:GFP panel). Western blottings in (A) and (B) used HSPB7 (for GST-HSPB7), GFP (for GFP and GFP-BAG3), and α-tubulin specific antibodies.
Table 2.
Gene-level association analysis of genes identified in the SNP-level analysis
Table 3.
DCM gene set association analysis