Table 1.
Subgrouping of medulloblastomas based on the evaluation of IHC immunoreactivity for selected protein markers [13].
Table 2.
Description of the patient cohort and patient-derived cell lines.
Table 3.
Antibodies used in this study.
Fig 1.
Immunohistochemical detection of selected protein markers in tumor samples.
Expression of β-catenin, filamin A, GAB1 and YAP1 in medulloblastoma tissue samples (1–6) as detected by immunohistochemistry. Bars, 100 μm.
Table 4.
Analysis of the expression and intracellular localization of selected protein markers in tumor samples and corresponding cell lines.
Fig 2.
Immunofluorescence detection of selected protein markers in corresponding cell lines.
Expression of β-catenin, filamin A, GAB1 and YAP1 in medulloblastoma cell lines as detected by IF. Each marker was visualized by indirect immunofluorescence using Alexa 488-conjugated secondary antibody (green); nuclei were counterstained with DAPI (blue). Bars, 50 μm (β-catenin, filamin A, YAP1) or 20 μm (GAB1).
Fig 3.
Immunoblot analysis of selected protein markers in medulloblastoma cell lines.
Expression of β-catenin, filamin A, GAB1 and YAP1 in patient-derived and reference medulloblastoma cell lines as detected by immunoblotting. β-actin served as a loading control.