Fig 1.
Overview of different subsets of samples analysed with fluorescence in situ hybridisation (n = 462), Nugent score (n = 527), G. vaginalis quantitative polymerase chain reaction (n = 528) and G. vaginalis sialidase quantitative polymerase chain reaction (n = 393).
Table 1.
Characteristics of vaginal samples.
Fig 2.
Superimposed confocal laser scanning microscopy images with 400x magnification of Gardnerella vaginalis biofilm, in three vaginal samples: vaginal epithelial cells DAPI in blue and G. vaginalis specific PNA-probe Gard162 with Alexa Fluor 647 in red.
2A shows an example of dispersed-only G. vaginalis (negative for biofilm), 2B shows a light G. vaginalis biofilm (a small number of bacteria are adhering to the vaginal epithelial cells) and 2C is an example of a heavy G. vaginalis biofilm (the vaginal epithelial cells are covered by bacteria).
Table 2.
The association between quantitative polymerase chain reaction results for G. vaginalis and G. vaginalis sialidase of vaginal samples and fluorescence in situ hybridisation and Nugent score results of vaginal slides.