Table 1.
Metabolic parameters of the platelet concentrates.
Fig 1.
Effect of 10-day storage on platelet activation, measured by light transmission aggregometry (LTA).
Aggregometry was measured in response to 10 μmol/L adenosine diphosphate (ADP, panel A), 4 μg/ml collagen (panel B) and 30 μmol/L thrombin receptor-activating peptide (TRAP-14, panel C). Light transmission in platelet concentrates (PCs) compared to autologous platelet poor plasma is expressed as percentage of maximal aggregation. Data are expressed as mean±SD and a dashed line was set on mean day 2—2SD. * = P<0.01, ** = P<0.001 compared to day 2.
Fig 2.
The effect of 10-day storage on spontaneous platelet activation by measuring P-selectin expression and activation of the GPIIb/IIIa receptor.
The percentage of positive cells (panel A and C for P-selectin and activated GPIIb/IIIa, respectively) and the median fluorescence intensity on the platelets (panel B and D for P-selectin and activated GPIIb/IIIa, respectively) are shown. Data are expressed as mean±SD and a dashed line was set on mean day 2 +2SD. * = P<0.01 compared to day 2.
Fig 3.
Effect of 10-day storage on platelet activation, measured by optimized flow-cytometric platelet activation test (PACT).
Adenosine diphosphate (ADP, panel A and D), collagen-related peptide (CRP, panel B and E) and thrombin receptor-activating peptide (TRAP-6, panel C and F) respectively, were used to activate platelets. The percentage cells that express P-selectin (panel A-C) and the median fluorescence intensity (MFI) of the P-selectin positive cells (panel D-F) are shown. Data are expressed as mean±SD and a dashed line was set on mean day 2 -2SD. * = P<0.01, ** = P<0.001 compared to day 2.