Fig 1.
Composition of diet fed to buffaloes on dry matter basis.
Table 1.
PCR primers for real time PCR assay with amplicon size and annealing temperature.
Table 2.
Effect of diets varying in TDN content on rumen pH in buffaloes.
Table 3.
Specific enzyme activity (Units/mg of protein) in buffaloes fed different levels of TDN.
Table 4.
Effect of diets varying in TDN content on VFA (mmol/dl), Ammonia nitrogen (mg/dl) and lactic acid (mg/dl) in the rumen of buffaloes.
Table 5.
Effect of diets varying in TDN content on microbial profile (log number of cells/ml RL) in the rumen of buffaloes.
Fig 2.
Individual rarefaction curve of metatranscriptomes of each sample of rumen liquor taken from buffaloes fed on different TDN (70, 85 and 100% of requirement) based diet.
Fig 3.
Shared phyla across different treatments.
Venn plot showing shared and unique phyla in rumen of buffaloes fed 70, 85 and 100% TDN. All taxa present within each group are plotted.
Fig 4.
Box plot indicating mean of ranked distances for most abundant five rumen phyla in buffaloes fed three levels of TDN.
Fig 5.
Per cent abundance of important bacteria in the rumen of buffaloes fed 70, 85, and 100% TDN of their requirement.
Fig 6.
Per cent abundance of total CAZy classes in rumen of buffaloes fed various levels of TDN.
Fig 7.
Per cent contribution of key cellulolytic bacteria in total GH and CBM CAZy families.
Table 6.
In vitro methane production (ml/gm DM) with rumen liquor of buffaloes fed different levels of TDN.
Table 7.
In Vitro methane production (ml/gm DDM) with rumen liquor of buffaloes fed different levels of TDN.
Table 8.
Volatile fatty acids (mmol/dl) in different feeds incubated with rumen liquor of buffaloes fed various levels of TDN (70, 85 and 100%).
Table 9.
In vitro ammonia nitrogen (mg/dl) in different feeds incubated with rumen liquor of buffaloes fed different levels of TDN.
Table 10.
In vitro true digestibility (IVTD %) of different feeds with rumen liquor of buffaloes fed different levels of TDN.