Table 1.
Rationale for protein selection.
Table 2.
Recombinant construct design, protein type and vaccine formulation.
Table 3.
Experimental setup presenting the different groups, sample size, mortality and relative percentage of survival (RPS).
Fig 1.
The cumulative mortality in rainbow trout experimental groups (replicates pooled) following infection challenge with A. salmonicida.
Asterisks (*) represent p values between groups (*p<0.05, **p< 0.01, ***p< 0.001).
Fig 2.
Levels of A. salmonicida-specific antibodies in serum measured by ELISA at 7 weeks post-immunization (wpi) and 3 weeks post- challenge (wpc).
Sera (diluted 1:500) were analyzed from 10 fish per group (5 fish per duplicate tank). Asterisks (*) represent p values (*p<0.05, **p< 0.01, ***p< 0.001) compared to saline control group.
Fig 3.
Levels of antibodies in serum of fish against individual VacB vaccine proteins.
Sera (diluted 1:100) was measured by ELISA from 10 fish per group (5 fish per duplicate tank) at 7 weeks post-immunization (wpi) (a) and at 3 weeks post-challenge (wpc) (b). Asterisks (*) represent p values (*p<0.05, **p< 0.01, ***p< 0.001) when compared to saline control group.
Fig 4.
Levels of antibodies in serum of fish against individual VacC vaccine proteins.
Sera (diluted 1:100) was measured by ELISA from 10 fish per group (5 fish per duplicate tank) at 7 weeks post-immunization (wpi) (a) and at 3 weeks post-challenge (wpc) (b). Asterisks (*) represent p values (* = P<0.05, ** = P< 0.01, *** = P< 0.001) when compared to saline control group.
Fig 5.
Correlation between mean antibody (diluted 1:100) levels for individual proteins at 7 weeks post-immunization (wpi) and survival in different experimental groups at 24 days post-challenge (dpc).
Antibody levels were measured from 10 fish per group (5 fish per duplicate tank) by ELISA one day before challenge.