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Fig 1.

Assessment of behavioural deficit for 6 months after stroke.

Basic Behavioural Test: A) Forelimb flexion and torso twisting. B) Total score over the timecourse of the experiment. C) Presence of a deficit (shaded) over the course of the experiment. Adhesive Removal Test: D) Placement and removal of tape on the forepaws. E) Time to remove contralateral (left) tape relative to baseline ability. F) Time to remove ipsilateral tape relative to baseline ability. Sunflower Seed Challenge: G) Intact and broken seeds. H) Number of whole seeds remaining. I) Number of pieces of seed.

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Fig 1 Expand

Table 1.

Scoring criteria for Basic Behavioural Test after stroke (Modified from Petullo et al)[19].

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Table 1 Expand

Table 2.

Summary of cohort sizes, CBF drop at MCAo and Tissue Damage (Mean±Standard Deviation).

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Table 2 Expand

Fig 2.

Ischemic damage over time after stroke.

A) Total volume of damage including cavity (shaded). B) Changes in hemispheric size C) Ischemic damage corrected for the effect of oedema and atrophy. All values Mean±Standard Deviation. Animals are represented by individual data points. * p<0.05

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Fig 2 Expand

Fig 3.

Evolution of ischemic related damage resulting from MCAo.

Representative images taken through the centre of the area of damage at approximately 1.7mm relative to bregma. A) Striatum. B) Cortex. Scale bar represents 50μm. Filled arrow head = red neuron; Open arrow head = hemorrhagic transformation; Arrow = Ghost neuron; Dotted line = striatal bundle; Star = Glial scar.

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Fig 3 Expand

Table 3.

Sample size calculations for behavioural and histological based outcome measures.

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Table 3 Expand