Table 1.
Comparison of growth characteristics of 52 B. burgdorferi sensu lato isolates cultured in MKP and BSK-H media according to numbers of spirochaetes and their morphology and motility.
Table 2.
Isolation of Borrelia burgdorferi sensu lato from skin samples of erythema migrans cultured in MKP and BSK-H media.
Fig 1.
Different plasmid profiles of paired strains isolated in MKP (M) and BSK-H (H) media.
MW, molecular weight marker. All isolated strains were identified as B. afzelii Mla1. Plasmid profiles of paired strains differ in the number of plasmids (significant for small plasmids under 48.5 kb) and MW (significant for large plasmids over 48.5 kb). Distinctions are evident in eight patients (ED, VS, ZM, SA, GV, BM, KM, and PM) and very likely in one patient (GB).
Fig 2.
Protein profiles of paired strains isolated in MKP (M) and BSK-H (H) medium from the skin of seven patients (TJ, ED, TZ, PM, ZD, LSB, and CD).
All isolated strains were identified as B. afzelii Mla1. MW, molecular weight marker; flagellin (F)–molecular weight about 41 kD; OspB (B)–molecular weight 34–36 kD; OspA (A)–molecular weight 30–33 kD, OspC (C)–molecular weight 23–27 kD.