Table 1.
Antibiotic resistance profiles for K. pneumoniae HS091147 and its transformant (T-pHS091147) in E. coli DH5α.
Fig 1.
Genes are color-coded dependent upon functional annotations as follows: pink, replication; blue, stability; green, propagation; orange, adaptation (the MDR region); grey, other functions and hypothetical proteins. The relaxase gene (traI) is indicated by the red bar. Red text highlights the resistance genes: blaKPC-2, blaTEM-1, blaCTX-M-14, dfrA25, sul1 and ΔqnrB2.
Fig 2.
Phylogenetic analysis of plasmid-encoded relaxase homologs.
Plasmid pHS091147 (in bold) and twelve other protein sequences were aligned, and the tree was generated with MEGA5 using the maximum-likelihood method. Solid black arrows point to the prototype plasmids for the MOBF12 and MOBF11 subfamilies. Other relaxase sequences of plasmids pKP048 (GenBank accession number FJ628167), pHS082416 (KF724507), pKHS2 (CP003224), pKP09085 (KF719970), pKPN4 (CP000649), pKPX-2 (AP012056), pK1HV (HF545434), F (AP001918), pSLT (AE006471), pED208 (AF411480), pET45 (CU468132) and R388 (BR000038) were obtained from GenBank.
Fig 3.
Comparative analysis of the multidrug-resistance region of plasmid pHS091147.
The relevant parts of pKP048, pKPHS2, SGI1-X and pTN48 are shown to highlight the syntenic regions. The horizontal line shown above or below the schematics (with one or both ends demarcated by solid triangles to indicated inverted repeats) represents intact, interrupted or truncated ISs, transposons and integrons as appropriate. Interruptions in the structures above are indicated as dashed lines. Regions with similar sequences are indicated in gray with corresponding percentages between the plasmids.