Fig 1.
Size distribution of miRNAs by high-throughput sequencing.
(a) Size distribution of ‘1005’ tea total miRNAs; (b) Size distribution of strain ‘1005’ conserved miRNAs; (c) Size distribution of strain ‘1005’ novel miRNAs.
Fig 2.
Gene ontology of the predicted targets.
Categorization of target genes was accomplished according to the cellular component (a), the molecular function (b) and biological process (c).
Table 1.
‘1005’ teamiRNA targets identified by degradome sequencing.
Fig 3.
The mRNA cleavage sites of degradome sequence.
Boxes indicate the cleavage sites; the numbers indicate the fraction of cloned PCR products terminating at different positions.
Fig 4.
The contents of catechin and expression levels of miRNAs and target genes in tea leaves of different maturity.
A, first leaves; B, third leaves; C, old leaves. The X axis in Fig 4 indicates the position of leaves; the Y axis in Fig 4a indicates percentages of catechin in dry tea leaves; the Y axis in Fig 4b-e indicates relative expression.
Fig 5.
Expression of miRNAs at different levels of maturity.
A, first leaves; B, third leaves; C, old leaves.
Fig 6.
The mRNA cleavage sites identified by 5’RLM-RACE.
Boxes indicate the cleavage sites; the numbers indicate the fraction of cloned PCR products terminating at different positions.
Fig 7.
The potential patterns of miRNAs that regulated cetechins synthesis in tea cultivar 1005.
The arrows pointing down represent down-regulating on catechins content.