Table 1.
Overview of sRNA sequencing data.
Fig 1.
Length distribution of sRNAs in the sRNA library of G. molesta.
A, The total number of clean and unique reads of sRNAs in lengths of 18–26 nt; B, the number of unique miRNAs ranging from 18–26 nt.
Fig 2.
Conservation analysis of 79 miRNA famililes within 33 species.
Conservation analysis of the 79 miRNA families identified from the sRNA library of G. molesta was conducted among 33 species belonging to the three phyla, Arthropoda, Nematoda and Chordata. The miRNA information of the other 32 species was retrieved from the miRBase. Colored box indicated the presence of the conserved miRNA family and the same color indicated species belonging to the same order of insect in Hexapoda, the same class in Arthropoda except for the Hexapoda species, C. elegans in Nematoda, and Homo sampiens in Chordata. The phylogenetic tree was constructed according to previous reports [48,49].
Table 2.
The most abundant conserved miRNAs in the sRNA library of G. molesta.
Fig 3.
Predicted secondary structures of the 44 novel miRNAs with middle to high abundance in the sRNA library of G. molesta.
The mature miRNAs located in the 5’ arm are shaded in yellow, whereas the mature miRNAs located in the 3’ arm are shaded in blue.
Table 3.
Novel miRNAs in G. molesta.
Fig 4.
Comparative analysis of qPCR detection with the expressional profiling in sRNA sequencing.
The y-axis indicates the abundance of miRNAs in the sRNA library and the relative transcriptional level of miRNAs detected using qPCR. The x-axis represents the specific assessed miRNAs, as 1: let-7, 2: mse92a, 3: bmo998, 4: pxy-6497-p5, 5: pxy-6497-p3, 6: bmo2b, 7: bmo281, 8: bmo279a, 9: bmo9a, 10: bmo305, 11: PC-5P-671, 12: PC-5P-1276, 13: PC-3P-1433, 14: PC-3P-4029, 15: PC-3P-16268, 16: PC-5P-35148, 17: PC-5P-15, 18: PC-5P-1018, 19: PC-5P-1258, 20: PC-3P-598.
Table 4.
miRNA cluster analysis in G. molesta.
Fig 5.
Network prediction of the circadian rhythm pathway in G. molesta.
The miRNAs in magenta and cyan circles respectively represent the conserved and novel miRNAs. Genes in yellow diamonds are clock genes and those involved in growth and development regulation. The regulatory network of miRNAs and their target genes for the circadian rhythm in G. molesta are linked with blue lines.
Table 5.
Information of candidate reference genes used for qRT-PCR.
Table 6.
Recommended reference genes for expressional qualification of microRNA in G. molesta under different experimental conditions.
Table 7.
Expressional profiles of let-7 in G. molesta quantified according to different normalizers.