Table 1.
Primers used in quantitative real-time polymerase chain reaction.
Fig 1.
Graphical length distribution summary of transcripts identified in S. olivacea transcriptome data sets.
Table 2.
Summary of assembly statistics.
Fig 2.
Similarity distribution of BLAST hits.
Fig 3.
Species distribution of top ten BLAST hits against UniProt database and D. melanogaster protein database, with a 1e-5 cut-off threshold.
Table 3.
Top 10 organism hits of assembled transcripts in S. olivacea transcriptome against UniProt database.
Table 4.
Top 20 annotations of S. olivacea transcriptome with the highest bit score.
Fig 4.
Transcript counts (log10) for Gene Ontology (GO) classification of the S. olivacea transcriptome for biological process (black), cellular component (white) and molecular function (grey) categories.
Table 5.
Number of hits of selected GO child terms and co-occurring terms based on multicellular organismal development (GO:0007275).
Table 6.
Selected regulators (i.e. proteins) of growth, sexual differentiation and reproduction expressed in the testis of S. olivacea.
Fig 5.
Distribution of putative single nucleotide polymorphisms (SNPs) in S. olivacea sequences.
Fig 6.
Heatmap of gene expression values depicting clustering of genes between immature (UMT_I, left panel) and mature stages (UMT_M, right panel) based on the expression of mRNAs for a set of significant genes (Padj < 0.05).
Sample names are represented in columns and significant genes are represented in rows. Genes are clustered together based on expression similarity. Low to high expression is represented by a change of colour from red to green, respectively. The colour key scale bar at upper left shows Z-score values for the heatmap.
Fig 7.
Heatmap of gene expression values depicting clustering of genes between immature (UMT_I, left panel) and mature stages (UMT_R, right panel) based on the expression of mRNAs for a set of significant genes (Padj < 0.05).
Sample names are represented in columns and significant genes are represented in rows. Genes are clustered together based on expression similarity. Low to high expression is represented by a change of colour from red to green, respectively. The colour key scale bar at upper left shows Z-score values for the heatmap.
Fig 8.
Heatmap of gene expression values depicting clustering of genes between immature (UMT_M, left panel) and mature stages (UMT_R, right panel) based on the expression of mRNAs for a set of significant genes (Padj < 0.05).
Sample names are represented in columns and significant genes are represented in rows. Genes are clustered together based on expression similarity. Low to high expression is represented by a change of colour from red to green, respectively. The colour key scale bar at upper left shows Z-score values for the heatmap.
Table 7.
Differential expression analysis of S. olivacea at gene level.
Table 8.
Selected differentially expressed genes between maturation stages in the S. olivacea testis expression profile.
Fig 9.
Fold difference of beta crystallin like gene in different maturation stages of testis of male S. olivacea. 18S rRNA was used as reference gene.
Different superscript letters within the same row indicate significant differences (P < 0.0001) between different stages of maturation.