Fig 1.
Sampling locations of 16 caves in Croatia.
Numbers refer to populations in Table 1. Insert (right) shows location of Croatia in Europe (black). Dotted area shows approximate range of P. anguinus. Drawing of P. anguinus is courtesy of Marija Crnčec.
Table 1.
List of sampling locations for detection of eDNA of Proteus anguinus in Croatia.
Fig 2.
60 bp long fragments produced with fluorescent labeled primers.
The blue peak indicates the species specific fragment for Proteus anguinus in positive control from tissue sample (A), in a sample collected with filtering 2 L of water (B) and in a sample collected in the solution composed of 1.5 mL of sodium acetate 3 M and 33 mL absolute ethanol (C).
Fig 3.
Posterior distributions of model parameters.
The figure shows the posterior distributions for availability and detectability probabilities for the two methods, filtration and precipitation.