Table 1.
Comparison of DNA yield and quality according to DNA collection method.
Fig 1.
Determination of AMY1 gene copy number by qPCR.
1–7 represents each of the 7 subjects recruited for this study, 8 represents the positive control known to have 14 diploid copies of AMY1 gene. The bars represent the different DNA samples extracted from blood, buccal swab and saliva of each individual. * denotes significant difference of p<0.01 (after bonferroni adjustment) between the different biological samples in each subject using ANOVA analysis.
Fig 2.
Determination of AMY1 gene copy number by ddPCR.
1–7 represents each of the 7 subjects recruited for this study, 8 represents the positive control known to have 14 diploid copies of AMY1 gene. The bars represent the different DNA samples extracted from blood, buccal swab and saliva of each individual.
Fig 3.
Comparison of AMY1 gene copy number determined by qPCR and ddPCR in different biological samples.
1–7 represents each of the 7 subjects recruited for this study, 8 represents the positive control known to have 14 diploid copy number of AMY1 gene. The bars represent the 2 different methods used to determine AMY1 gene copy number in the samples (qPCR and ddPCR). A: Comparison of AMY1 gene copy number in blood. B: Comparison of AMY1 gene copy number in buccal swab. C: Comparison of AMY1 gene copy number in saliva. * denotes significant difference of p<0.01 between qPCR and ddPCR for each type of biological sample after paired Student’s t test analysis.