Fig 1.
12-week-old, female, American bulldog with ARCI.
Top left: erythema of the rostral muzzle with scaling. Top right: generalized soft white scale is present on the entire body. Bottom left: The abdomen is erythematous with tightly adherent light brown scale. Bottom right: Paw pad hyperkeratosis with yellowish discoloration.
Table 1.
PCR primers and conditions used in sequencing the canine NIPAL4 gene.
Fig 2.
Sequencing chromatograms of a region within exon 6 of canine NIPAL4.
Comparison of the sequences from the normal dog (upper panel) and from the dog affected with ARCI (lower panel) show a missing cytosine (arrow) in the affected dog.
Fig 3.
PCR-RFLP based genotyping results and corresponding pedigree of an ABD family with affected ARCI dogs.
Four clinically affected dogs (8, 11, 13, and 14) were homozygous for the variant allele, their clinically healthy sire (6), dam (15) and a littermate (12) were heterozygous, and 3 littermates were homozygous for the wild type allele (7, 9, and 10). (M = marker, squares = males, circles = females, open symbols = clinically normal, filled in symbols = clinically affected). The p value for the association of homozygosity for the variant allele with the clinical phenotype (affected versus non-affected) is 0.0003 by the Fisher Exact Probability Test.