Fig 1.
The read (X)-, phase (Y)- and slice (Z)-encoding directions with respect to the anatomical orientation of the rat brain (L, left; R, right; S, superior; I, inferior; P, posterior; and A, anterior) in coronal, axial and sagittal acquisitions.
Fig 2.
The four voxel geometries used in the acquisitions with respect to the coronal, axial and sagittal views of the rat brain.
The isometric voxel is shown in blue, the anisometric voxel in the PA direction in green, the anisometric voxel in the LR direction in red and the anisometric voxel in the SI direction in yellow.
Table 1.
Anisometric voxel orientation.
Table 2.
Whole-brain DTI parameters.
Fig 3.
Whole-brain histograms of the FA for the different acquisition orientations with isometric and anisometric voxels.
Fig 4.
Boxplot of the average FA values of all the regions of the template for the different acquisitions.
On each box, the central mark is the median, the black dot the mean, the edges of the box are the 25th and 75th percentiles, the whiskers extend to the most extreme data points and the outliers are plotted individually as red crosses. Asterisks indicate significant difference (p<0.05) between anisometric and isometric acquisitions acquired in the same orientation (coronal, axial or sagittal).
Table 3.
Number of regions showing significant.
Fig 5.
Regions of interest segmented in the right hemisphere and the mean and standard deviation of FA for each region.
Regional average FA values in the coronal isometric, coronal aniPA, axial aniSI and sagittal aniLR acquisitions. The (a) caudate putamen, (b) internal capsule, (c) corpus callosum, (d) anterodorsal hippocampus, (e) dorsolateral thalamus and (f) somatosensory cortex. Asterisks show significant differences (p<0.05) in relation to the isometric acquisition.
Fig 6.
Coronal, axial and sagittal views of the FA color map.
Acquisitions in the three orientations using isometric and anisometric voxels in the slice-encoding direction. The white lines indicate the corpus callosum (cc) and cerebellum (cb) locations for anatomic reference.
Fig 7.
Orientation distribution functions (ODF).
Magnification of the same anatomical region (red square) from the coronal view for acquisitions obtained with different orientations and isometric and anisometric voxels in the slice-encoding direction. The white box shows an area where differences between acquisitions can be observed.
Fig 8.
Sagittal view of the corticospinal tract streamlines.
Deterministic tractography based on CSD for acquisitions in different orientations and with isometric and anisometric voxels in the slice-encoding direction. Note that in the sagittal anisometric acquisition there are not fibers connecting both regions of interest. In this case, fibers crossing any of the regions have been plotted.
Table 4.
Streamline measurements.
Fig 9.
(a) FA-weighted and (b) FN-weighted connectomes.
Estimation was undertaken from the acquisitions in the three orientations and with isometric and anisometric voxels in the slice-encoding direction.
Fig 10.
Graph metrics of the binary, FA-weighted and FN-weighted connectomes.
Estimation was performed from the three isometric and the three anisometric voxels in the slice-encoding direction acquisitions.