Fig 1.
Infrared spectra of fungal biomass before and after lipid extraction.
FTIR spectra recorded of Mucor circinelloides (A) and Mortierella alpina (B) biomass before (BE) and after extraction of lipids using the methods of Bligh, Folch and Lewis.
Fig 2.
Infrared spectra of all extraction phases after Folch extraction.
Lipids were extracted from Mortierella alpina biomass using the Folch method and infrared spectra were recorded of the biomass before (BE) and after extraction (AE), the extract phase (EP) and the water phase (WP).
Table 1.
Lipid extraction yield after fungal biomass extraction using the methods of Bligh, Folch and Lewis and different biomass pretreatments.
Fig 3.
Infrared spectra of M.circinelloides biomass after acid hydrolysis pretreatment and lipid extraction.
Mucor circinelloides biomass was subjected to acid hydrolysis and infrared spectra of the biomass were recorded before (BE) and after pretreatment and extraction of lipids using the methods of Bligh, Folch and Lewis.
Fig 4.
Infrared spectra of fungal biomass after bead beating pretreatment and lipid extraction.
Mucor circinelloides (A) and Mortierella alpina (B) biomass was subjected to bead beating pretreatment and infrared spectra were recorded of the biomass before (BE) and after extraction of lipids using the methods of Bligh, Folch and Lewis. Bead beating exposure time was 1 min.
Table 2.
Quantification of lipids in fungal biomass using gravimetric, internal standard and external calibration approaches.