Fig 1.
Cell morphology differences between A549 and A549/DDP cells.
Micrographs showing A549 and A549/DDP cells cultured for 3 days in the absence or presence of cisplatin (10μg/mL).
Fig 2.
The inhibitory effects of different concentrations of cisplatin (DDP) on A549 and A549/DDP cells.
Cell viability, as assessed by the CCK-8 assay, was determined 24 h after exposure of A549 or A549/DDP cells to increasing amounts of cisplatin. Results represent the average of triplicate wells and are representative of three independent experiments. Black bars and symbols, A549/DDP; red bars and symbols, A549.
Fig 3.
Distribution of the differentially expressed genes shown as a volcano plot.
Each point represents a gene. The green dots represent the down-regulated differentially expressed genes, red dots represent the up-regulated differentially expressed genes, and black dots represent non-differentially expressed genes.
Table 1.
Statistical summary of transcriptome sequencing.
Table 2.
Statistical summary of the mapping results.
Fig 4.
Annotation statistics of differentially expressed genes in the secondary node of GO. The horizontal axis shows the secondary nodes of three categories in GO. The vertical axis displays the percentage of annotated genes versus the total gene number. The red columns display annotation information of the total genes and the blue columns represent annotation information of the differentially expressed genes only.
Fig 5.
COG function classification of the consensus sequences.
The COG categories are shown on the horizontal axis and gene numbers and proportions are plotted on the vertical axis.
Fig 6.
KEGG categories of differentially expressed genes.
The vertical axis lists the names of the metabolic pathways in the KEGG database, and the horizontal axis shows the proportion of annotated genes in each pathway versus the total number of annotated genes.
Fig 7.
Significant deregulated key signaling nodes of most DEGs enriched pathways.
Significant deregulated key signaling nodes of PI3K/AKT, MAPK, Focal Adhesion and Actin Cytoskeleton pathway.
Fig 8.
Correlation of BIRC2 or BIRC3 expression and DFS of patients with lung adenocarcinoma.
Low expression of BIRC2 or BIRC3 is associated with improved DFS. Green and blue lines indicated low and high expression groups, respectively. P <0.05 was considered to be statistically significant.
Fig 9.
The differentially expressed genes detected by transcriptome sequencing confirmed by qRT-PCR.
qRT-PCR was performed for 5 genes that were identified to be differentially expressed between A549 and A549/DDP cells. The expression level of each gene was normalized to the level in A549 cells. A–E: FGF13, FGF12, ITGAD, CACNG4, and RELB, respectively.
Fig 10.
KEGG categories of HepG2/DDP cells DEGs.
The vertical axis lists the names of the metabolic pathways in the KEGG database, and the horizontal axis shows the proportion of annotated genes in each pathway versus the total number of annotated genes.